Journal: Acta Histochemica et Cytochemica

Article Title: Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload

doi: 10.1267/ahc.23-00041

Figure Lengend Snippet: The expression patterns of cyclin D3 in satellite cell (SC) nuclei of overloaded muscles. The line graph indicates time course changes in the percentage of myofibers with SC nuclei expressing cyclin D3. Values are the mean ± SD. Note: in the contralateral sham-operated muscles, neither cyclin D3 nor myogenin expression was detected in the nuclei of SCs throughout the experimental periods. * Significantly different compared with post-surgery day 1 and 3 muscles (p < 0.05).

Article Snippet: The primary antibodies used in the present study were as follows; rabbit polyclonal anti-laminin (a marker molecule for the basement membrane; 1:3000; Sigma, St Louis, MO), goat polyclonal anti-M-cadherin (a previously determined SC marker molecule [ , ]) (1:200; Santa Cruz Biotech., Santa Cruz, CA), mouse monoclonal anti-cyclin D3, rabbit polyclonal anti-dystrophin (a marker molecule for the plasma membrane) (1:100; NeoMarkers, Fremont, CA, USA), rabbit polyclonal anti-myogenin (1:100; Santa Cruz Biotech.), mouse monoclonal anti-myogenin (1:100; BD PharMingen, San Diego, CA, USA), rabbit polyclonal anti-cyclin-dependent kinase inhibitor p21 (Ab-5, 1:100; Oncogen Research Products, Cambridge, MA, USA) and rabbit polyclonal anti-Ki67 (1:100; Novocastra, Newcastle upon Tyne, UK).

Techniques: Expressing, Muscles

Journal: Acta Histochemica et Cytochemica

Article Title: Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload

doi: 10.1267/ahc.23-00041

Figure Lengend Snippet: The expression patterns of myogenin in satellite cell (SC) nuclei in overloaded muscles. In post-surgery day 7 overloaded muscles, immunostaining was performed to visualize the localization of myogenin ( a ), M-cadherin ( b ), nuclei ( c ), and the three merged images ( d ). The arrows in ( a ), ( c ), and ( d ) indicate myogenin-positive nuclei expressed in an M-cadherin-positive SC. Bar = 30 μm. ( e ) time course changes in the percentage of myofibers with SC nuclei expressing myogenin in overloaded muscles. Values are the mean ± SD. Note: in the contralateral sham-operated muscles, neither myogenin nor myogenin expression was detected in the nuclei of SCs throughout the experimental periods. *Significantly different compared with post-surgery days 1, 3, and 5 in overloaded muscles (p < 0.05).

Article Snippet: The primary antibodies used in the present study were as follows; rabbit polyclonal anti-laminin (a marker molecule for the basement membrane; 1:3000; Sigma, St Louis, MO), goat polyclonal anti-M-cadherin (a previously determined SC marker molecule [ , ]) (1:200; Santa Cruz Biotech., Santa Cruz, CA), mouse monoclonal anti-cyclin D3, rabbit polyclonal anti-dystrophin (a marker molecule for the plasma membrane) (1:100; NeoMarkers, Fremont, CA, USA), rabbit polyclonal anti-myogenin (1:100; Santa Cruz Biotech.), mouse monoclonal anti-myogenin (1:100; BD PharMingen, San Diego, CA, USA), rabbit polyclonal anti-cyclin-dependent kinase inhibitor p21 (Ab-5, 1:100; Oncogen Research Products, Cambridge, MA, USA) and rabbit polyclonal anti-Ki67 (1:100; Novocastra, Newcastle upon Tyne, UK).

Techniques: Expressing, Muscles, Immunostaining

Journal: Acta Histochemica et Cytochemica

Article Title: Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload

doi: 10.1267/ahc.23-00041

Figure Lengend Snippet: Photomicrographs of post-surgery day 7 overloaded muscles showing the relationship between myogenin and p21 in a satellite cell (SC) nucleus. Immunostaining was performed to visualize the localization of cyclin D3 ( a ), myogenin ( b ), and nuclei ( c ), and the three images merged ( d ). The arrows in ( a )–( d ) indicate the coexpression of cyclin and myogenin in an SC. Immunostaining was performed to visualize the localization of cyclin D3 ( e ), p21 ( f ), and nuclei ( g ), and the three images merged ( h ). The arrows in ( e )–( h ) indicate myogenin and p21 coexpression in an SC. Immunostaining was performed to visualize the localization of myogenin ( i ), p21 ( j ), and nuclei ( k ), and the three images merged ( l ). The arrows in ( i )–( l ) indicate myogenin and p21 coexpression in a SC. Bar = 30 μm.

Article Snippet: The primary antibodies used in the present study were as follows; rabbit polyclonal anti-laminin (a marker molecule for the basement membrane; 1:3000; Sigma, St Louis, MO), goat polyclonal anti-M-cadherin (a previously determined SC marker molecule [ , ]) (1:200; Santa Cruz Biotech., Santa Cruz, CA), mouse monoclonal anti-cyclin D3, rabbit polyclonal anti-dystrophin (a marker molecule for the plasma membrane) (1:100; NeoMarkers, Fremont, CA, USA), rabbit polyclonal anti-myogenin (1:100; Santa Cruz Biotech.), mouse monoclonal anti-myogenin (1:100; BD PharMingen, San Diego, CA, USA), rabbit polyclonal anti-cyclin-dependent kinase inhibitor p21 (Ab-5, 1:100; Oncogen Research Products, Cambridge, MA, USA) and rabbit polyclonal anti-Ki67 (1:100; Novocastra, Newcastle upon Tyne, UK).

Techniques: Muscles, Immunostaining

Journal: Acta Histochemica et Cytochemica

Article Title: Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload

doi: 10.1267/ahc.23-00041

Figure Lengend Snippet: Photomicrographs of post-surgery day 5 overloaded muscles showing the coexpression of cyclin D3, myogenin, and p21. Triple fluorescence staining to visualize the localization of cyclin D3 ( a ), myogenin ( b ), p21 ( c ), and nuclei ( d ) were performed. An adjacent serial section of post-surgery day 5 overloaded muscle was prepared at 5 μm thickness and immunostained for cyclin D3/myogenin/p21 and with DAPI. Arrows ( a )–( d ) indicate coexpression of cyclin D3, myogenin and p21-positive immunoreactivities in a nucleus. Bar = 30 μm.

Article Snippet: The primary antibodies used in the present study were as follows; rabbit polyclonal anti-laminin (a marker molecule for the basement membrane; 1:3000; Sigma, St Louis, MO), goat polyclonal anti-M-cadherin (a previously determined SC marker molecule [ , ]) (1:200; Santa Cruz Biotech., Santa Cruz, CA), mouse monoclonal anti-cyclin D3, rabbit polyclonal anti-dystrophin (a marker molecule for the plasma membrane) (1:100; NeoMarkers, Fremont, CA, USA), rabbit polyclonal anti-myogenin (1:100; Santa Cruz Biotech.), mouse monoclonal anti-myogenin (1:100; BD PharMingen, San Diego, CA, USA), rabbit polyclonal anti-cyclin-dependent kinase inhibitor p21 (Ab-5, 1:100; Oncogen Research Products, Cambridge, MA, USA) and rabbit polyclonal anti-Ki67 (1:100; Novocastra, Newcastle upon Tyne, UK).

Techniques: Muscles, Fluorescence, Staining